diff --git a/kernel/api/workspace.go b/kernel/api/workspace.go
index 3735e01bc..00bc305a8 100644
--- a/kernel/api/workspace.go
+++ b/kernel/api/workspace.go
@@ -38,6 +38,11 @@ func listWorkspaceDirs(c *gin.Context) {
 	userHomeConfDir := filepath.Join(util.HomeDir, ".config", "siyuan")
 	workspaceConf := filepath.Join(userHomeConfDir, "workspace.json")
 	data, err := os.ReadFile(workspaceConf)
+	if nil != err {
+		logging.LogErrorf("read workspace conf [%s] failed: %s", workspaceConf, err)
+		return
+	}
+
 	var workspacePaths []string
 	if err = gulu.JSON.UnmarshalJSON(data, &workspacePaths); nil != err {
 		logging.LogErrorf("unmarshal workspace conf [%s] failed: %s", workspaceConf, err)
diff --git a/kernel/util/working.go b/kernel/util/working.go
index f5dba1705..69829e60a 100644
--- a/kernel/util/working.go
+++ b/kernel/util/working.go
@@ -219,6 +219,7 @@ func initWorkspaceDir(workspaceArg string) {
 
 		tmp := workspacePaths[:0]
 		for _, d := range workspacePaths {
+			d = strings.TrimRight(d, " \t\n") // 去掉工作空间路径尾部空格 https://github.com/siyuan-note/siyuan/issues/6353
 			if gulu.File.IsDir(d) {
 				tmp = append(tmp, d)
 			}